Rapid Determination of Urinary Total Porphyrins by Ion Exchange Chromatography
نویسندگان
چکیده
منابع مشابه
Rapid determination of urinary total porphyrins by ion exchange chromatography.
Total urinary porphyrins were eluted from a commercially available anion exchange Chromatographie column with hydrochloric acid and determined spectrophotometrically. For 1 ml samples the optimum concentration range for measurement was found to lie between 0.1 and 1.5 mg of total porphyrin/1 urine. Assay of a single sample takes 12 min. The specificity (measurement at three wave lengths), sensi...
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A procedure has been developed for the assay of urinary porphyrins. Total porphyrins are extracted from acidified urine into n-butanol. The fluorescence of this solution under ultraviolet illumination gives a preliminary indication of abnormal levels of porphyrins. They are then separated and identified by thin-layer chromatography. Uroporphyrin and coproporphyrin can be qualitatively estimated...
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Ion chromatography was used to determine urinary oxalate concentration. The minimal detectable limit in a standard solution was 0.02 microgram/ml, and the regression line for the standard curve from 0.5 to 10 micrograms/ml had a significant correlation coefficient (p less than 0.01), but it was difficult to obtain reproducible results with ion chromatography. There were two obstacles: Urine was...
متن کاملTitle Determination of urinary oxalate by ion chromatography: some modifications
Ion chromatography was used to determine urinary oxalate concentration. The minimal detectable limit in a standard solution was 0.02 flg/ml, and the regression line for the standard curve from 0.5 to 10 flg/ml had a significant correlation coefficient (p<O. 01), but it was difficult to obtain reproducible results with ion chromatography. There were two obstacles: Urine was always oversaturated ...
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A method was established for the simultaneous determination of germanium and tin in one run by ion chromatography. Using a selected eluent, Ge and Sn were separated on a bifunctional ion-exchange column, followed by a postcolumn chromogenic reaction and spectrophotometric detection at 520 nm. The whole procedure was completed within 5 min with high sensitivity and selectivity at the low-/zg/1 l...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1971
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1971.9.5.415